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Effect of divalent ions and EDTA on the activity of UDP(βS)-GlcNAc and UDP-GlcNAc. Numbers in parenthesis are standard deviations of three parallel experiments.
| Cations (10 mM) | Relative Activity (%) UDP-GlcNAc |
Relative Activity (%) UDP(βS)-GlcNAc (2) |
|---|---|---|
| Mn2+ | 100 | 100 |
| Ca2+ | 115.9 (11.2) | 12.5 (1.33) |
| Mg2+ | 98.6 (7.9) | 92.6 (2.3) |
| Fe2+ | 51.2 (2.2) | 5.7 (0.98) |
| Mo2+ | 35.5 (3.62) | 21.7 (1.1) |
| Pd2+ | 21.5 (2.2) | 3.9 (0.55) |
| Co2+ | 8.0 (0.32) | 5.6 (1.28) |
| Cu2+ | <1 | <1 |
| Hg2+ | <1 | <1 |
| Ni2+ | <1 | <1 |
| Zn2+ | <1 | <1 |
| EDTA | <1 | <1 |
