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. Author manuscript; available in PMC: 2012 Sep 14.
Published in final edited form as: Carbohydr Res. 2011 May 12;346(12):1576–1580. doi: 10.1016/j.carres.2011.05.005

Table 2.

Summary of kinetic data for LgtA catalyzed reaction using azido modified lactose and UDP(βS)-GlcNAc and UDP-GlcNAc as substrates. a: azido modified lactose; b: donor. The number is reported as the average of three parallel experiments. The number in parenthesis shows the standard error.

Ka,Apparant (mM) Kia (mM) Kb (mM) Vmax (mM min−1) [E0] (mg mL−1)
UDP(βS)-GlcNAc (2) 6.08 (0.93) 15.0 (2.75) 51.5 (4.3) 0.211 (0.194) 1.63
UDP-GlcNAc 5.84 (0.71) 14.8 (1.18) 0.40 (0.22) 0.456 (0.131) 0. 204