FIG. 3.

Both Kin28 and Srb10 can promote transcription. (A) Nuclear extracts (NE) made from wild-type, Kin28-as, Srb10-as, or Kin28-as Srb10-as strains were used as indicated. The transcription reactions were performed as described in Materials and Methods. Quantitation of the results is shown in the lower panel. Transcription activity of each nuclear extract without inhibitor was normalized to 100%. (B) Transcription reaction performed as described for panel A using immobilized templates and NA-PP1 with extracts from the indicated strains. In lane 9, a saturating amount of purified wild-type CAK was added.