FIG. 4.

Mnt knockdown triggers apoptosis. (A) The indicated cells were shifted to medium containing 0.1% fetal calf serum, and their viability was determined by trypan blue dye exclusion. A representative experiment performed in triplicate is shown. (B) Apoptosis was determined by FACS analysis of annexin V-positive, propidium iodide-stained cells 48 h after serum depletion. The percentages of annexin V-positive cells are given in the quadrants (early apoptotic, lower right panels; late apoptotic, upper right panels). PE, phycoerythrin.