FIG. 5.

Phenotypes observed in MEFs on Mnt knockdown are not due to general effects of expressing shRNAi. (A) Immunoblot analyses for Mnt of MEFs infected with control (MSCV-pBlueRNAi-IRES-GFP) or three different MSCV-MntRNAi-IRES-GFP-expressing retroviruses. β-Actin levels were assessed as a control. (B) MEFs expressing any of the three MSCV-MntRNAi-IRES-GFP retroviruses displayed accelerated rates of proliferation compared to MEFs expressing the MSCV-pBlueRNAi-IRES-GFP retrovirus. (C) MEFs expressing any of the three MSCV-MntRNAi-IRES-GFP retroviruses, but not those infected with the retrovirus encoding MSCV-pBlueRNAi-IRES-GFP, undergo rapid apoptosis following serum withdrawal.