FIG. 6.

Mnt knockdown provokes transformation in conjunction with activated Ha-Ras. (A) A representative field of soft-agar assays of MEFs expressing GFP, GFP plus Ras, MntRNAi, and MntRNAi plus Ras are shown. MntRNAi-expressing MEFs were capable of growing in an anchorage-independent manner, although colonies were reduced 10- to 20-fold in their numbers and were much smaller than colonies generated by MEFs expressing both Ha-Ras and MntRNAi. (B) MEFs expressing both Ras and MntRNAi are tumorigenic in immunocompromised Nu/Nu mice (n = 5 for each group). BALB/c-3T3 fibroblasts expressing MntRNAi alone were also tumorigenic in this assay (n = 5).