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. 2012 Jul 2;13:293. doi: 10.1186/1471-2164-13-293

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Copyright ©2012 Zhuang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Southern blot analysis of Atlantic cod genomic DNA showing presence of AFGP coding sequences.TaqI digested genomic DNA (~10–15 μg) from Atlantic cod (lanes 1–14) and polar cod (lanes 15–17) hybridized strongly to a polar cod B. saida AFGP coding sequence probe. Atlantic cod individuals include Norwegian coastal cod (NCC) and North East Arctic cod (NEAC) from the Finnmark coast and marginal Barents Sea sites: (lanes 1–3) N69° 26.91′ E19° 37.56′; (lanes 4–5) N69° 58.34′ E30° 2.37′; (lanes 6–8) N70° 7.24′ E30° 48.47′; (lanes 9–13) N71° 11.93′ E27° 59.29′, and one individual (lane 14) from Øresund, Denmark. NEAC and NCC are distinguished by their PanI genotype, BB and AA respectively as indicated, while AB can either be NEAC or NCC. For comparison, the related freshwater cod Lota lota (lane 18) that does not have AFGP shows no hybridization.