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. 2012 Jun 21;13:19. doi: 10.1186/1471-2199-13-19

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Copyright ©2012 Gomar-Alba et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Yhr087wp partially associates with ribosomes. Whole cell extracts obtained from a strain containing an Yhr087wp-TAP-tagged protein were fractionated as described in the Materials and Methods section. The Figure shows the distribution of protein Yhr087wp between the cytoplasmic (CF) and ribosomal (RF) fractions by Western analysis. A sample corresponding to the whole cell extract (WCE) is also included. As a control of the fractionation procedure, the location of a cytosolic (α-tubulin) and a ribosomal protein (Rpl5p) was determined by Western analysis using specific antibodies against these proteins.