FIG. 2.

Defective JNK and ERK induction by EGF in Gab1-deficient cells. Subconfluent fibroblasts were starved in serum-free DMEM for 36 h, stimulated with EGF (100 ng/ml) for 0, 1, 5, 10, 20, or 30 min, and lysed with lysis buffer. Equal amounts of cell lysates (50 μg of total proteins) were resolved on a SDS-polyacrylamide gel and immunoblotted (IB) by the indicated antibodies. +/+, wild-type cells; −/−, Gab1^−/− cells.