FIG. 9.

Sensitivity of bik^−/− bcl-2^−/− lymphocytes to apoptotic stimuli. (A) bik^−/− bcl-2^−/− T lymphocytes were cultured in the absence of cytokines, and viability was assessed by measuring the PI uptake after 24 and 48 h. Lymphocytes were sorted from lymph nodes of Ly5.1 recipients reconstituted with Ly5.2 wt (n = 5), bik^−/− bcl-2^+/+ (n = 3), bik^+/+ bcl-2^−/− (n = 4), and bik^−/− bcl-2^−/− (n = 5) fetal liver HSC. (B) bik^−/− bcl-2^−/− B lymphocytes were cultured in the absence of cytokines, and viability assessed as described in the legend to panel A.