TABLE 1.
Effector dissociation constantsa for BenM and BenM-EBD
| Effector |
Kd (mM)b
|
|
|---|---|---|
| BenM | BenM-EBD | |
| Benzoate | 1.2 ± 0.2 | 1.1 ± 0.1 |
| Muconate | 0.28 ± 0.05 | 0.12 ± 0.02 |
| Muconate + 1 mM benzoate | 0.49 ± 0.09 | 0.20 ± 0.03 |
a
The dissociation constants for the protein-effector complexes were determined with the following equation and the Sigmaplot 2000 software hyperbolic regression function (SPSS Inc.): ΔVoltsobs = (ΔVoltsmax [E]/Kd[E]) + c[E]. In this equation, ΔVoltsobs is the observed change in voltage, [E] is the effector concentration, Kd is the dissociation constant for the effector, and c accounts for the inner-filter effect of the effector on the fluorescence signal.
b
The values shown are averages of at least three experiments.