FIG. 5.

RACE products and associated transcriptional start sites. (A) Diagram showing elements of the 5′-RACE experiment. Vertical arrows represent the locations of putative promoters, named according to the relative position of the transcriptional start sites. Open arrows represent putative gearbox promoters, and the closed arrow represents the single putative σ⁷⁰ promoter. Horizontal arrows represent the location of the reverse primers which were used. (B) Agarose gel electrophoresis of RACE products. DNA was resolved on an agarose gel. Lanes 1 and 5, 100-bp ladder (New England Biolabs). Major bands, from top to bottom, represent PCR products that initiate at cDNA 5′ ends (transcriptional start sites), as follows: lanes 2 and 3, TS-4267, TS-4055, and TS-3939; lane 4, TS-3939; lane 6, TS-4267, TS-4493 and unknown; lane 7, TS-4267; lane 8, TS-4055; lane 9, TS-3626; lane 10, TS-3492 and unknown.