TABLE 2.
Bacterial strains used in this study
| Strain | Relevant phenotype, genotype, or description | Source or reference |
|---|---|---|
| E. coli DH5αMCR | F−mcrA Δ(mrr-hsdRMS-mcrBC) endA1 supE44 thi-1 recA1 gyrA relA1 Δ(lacIZYA-argF)U169 deoRφ80dlacΔ(lacZ)M15 | BRLa |
| N. gonorrhoeae | ||
| F62 | P. F. Sparlingb | |
| F62ΔlgtA | 239-bp ApoI deletion in lgtA; this strain produces L8LOS | 47 |
| F62ΔlgtAΔlgtE | 622-bp BspEI-to-AgeI deletion in lgtE of the parent strain F62ΔlgtA | 47 |
| F62ΩBsrGI | F62 containing the Ω interposon at the BsrGI site between the stop codon of glySβ and the start codon of lgtA | This study |
| F62ΩAscI | F62 containing the Ω interposon within the AscI site of lgtB | This study |
| F62ΩClaI | F62 containing the Ω interposon within the ClaI site of lgtC | This study |
| F62ΩNruI | F62 containing the Ω interposon within an engineered NruI site in the polyguanine region of lgtD | This study |
| F62ΩEcoRV | F62 containing the Ω interposon within the EcoRV site of lgtD | This study |
| F62ΩEcoRI | F62 containing the Ω interposon within an engineered EcoRI site between lgtD and lgtE; the termination codon of lgtD contains part of the EcoRI recognition sequence, and the last base of the recognition sequence is 18 bp from the lgtE start codon | This study |
| F62LgtA(xylE) | F62 with the xylE reporter gene inserted at the BsrGI site 58 bases before the start codon of lgtA | This study |
| F62LgtE(xylE) | F62 with the xylE inserted into lgtE | This study |
| F62LgtE(xylE)ΩBsrGI | F62LgtE(xylE) containing the Ω interposon at the BsrGI site 58 bases before the start codon of lgtA | This study |
| F62LgtE(xylE)ΩAscI | F62LgtE(xylE) containing the Ω interposon within the AscI site of lgtB | This study |
| F62LgtE(xylE)ΩClaI | F62LgtE(xylE) containing the Ω interposon within the ClaI site of lgtC | This study |
| F62LgtE(xylE)ΩNruI | F62LgtE(xylE) containing the Ω interposon within an engineered NruI site in the polyguanine region of lgtD | This study |
| F62LgtE(xylE)ΩEcoRV | F62LgtE(xylE) containing the Ω interposon within the EcoRV site of lgtD | This study |
| F62LgtE(xylE)ΩEcoRI | F62LgtE(xylE) containing the Ω interposon within an engineered EcoRI site between lgtD and lgtE | This study |
a
BRL, Bethesda Research Laboratories.
b
University of North Carolina, Chapel Hill.