FIG. 3.

(A) Primer extension analysis of clpB expression at 37°C (lanes 1 and 2) or following a 10-min heat shock at 42°C (lanes 3 and 4). Total RNA (20 μg) extracted from L. monocytogenes L028 (lanes 1 and 3) and LM2001 (ΔctsR) (lanes 2 and 4), was used as a template for reverse transcriptase. The corresponding DNA sequence is shown on the left. (B) Nucleotide sequence of the L. monocytogenes L028 clpB promoter region. Potential −35 and −10 promoter sequences are overlined; the transcriptional start site is indicated by +1; the CtsR direct-repeat operator sequence is indicated by arrows; the potential RBS sequence is underlined; the translational start site is boxed, and the deduced amino acid sequence is indicated below the nucleotide sequence.