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. 2004 Feb;186(4):1165–1174. doi: 10.1128/JB.186.4.1165-1174.2004

FIG. 4.

FIG. 4.

(A) CtsR binds specifically to the clpB promoter region. DNA-binding reactions were performed with radiolabeled DNA fragments (10,000 cpm) corresponding to the clpB promoter region. Lane 1, no protein; lane 2, 7 ng; lane 3, 70 ng; lane 4, 700 ng. (B and C) DNase I footprinting analysis of CtsR binding to the clpB promoter region. Each lane contains 50,000 cpm of radiolabeled DNA fragment corresponding to the nontemplate strand (panel B) or the template strand (panel C) of the L. monocytogenes clpB promoter region. Fragments were incubated with increasing amounts of purified CtsR. Lane 1, no protein; lane 2, 35 ng; lane 3, 350 ng; lane 4, 3,500 ng; lane 5, Maxam and Gilbert reactions of the corresponding DNA fragment. Brackets indicate regions protected by CtsR. (D) Nucleotide sequence of the clpB promoter region. The DNase I protected area is boxed, and arrows indicate the CtsR direct-repeat recognition sequence. Positions are numbered relative to the translational initiation codon.