FIG. 3.

RT-PCR analysis of bxlE, bxlF, bxlG, and bxlA RNA. (A) The schematic locations of oligonucleotide primers (P5 to P13) used in RT-PCR analysis are shown (for more details, see Table 1). The primer P5 was used for RT reactions. (B) Agarose gel electrophoresis of RT-PCR products. RT-PCR analysis was performed with total RNA isolated from S. thermoviolaceus OPC-520 in medium containing 1% xylan (lanes 1, 4, 7, and 10), 1% xylobiose (lanes 2, 5, 8, and 11), or 1% glucose (lanes 3, 6, 9, and 12). Lanes: 1, 2, and 3, cDNA products by P6 and P7; 4, 5, and 6, cDNA products by P8 and P9; 7, 8, and 9, cDNA products by P10 and P11; 10, 11, and 12, cDNA products by P12 and P13; M, molecular size standards.