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. 2004 Feb;186(4):1029–1037. doi: 10.1128/JB.186.4.1029-1037.2004

TABLE 1.

Sequences of primers used in this study

Primer Sequencea Location
P1 5′-CCGGTCTGAGCTCCTGCGGGTC-3′ 3957-3978b
P2 5′-GCCCGTCCCTGCAGTCTCACCG-3′ 5262-5241b
P3 5′-GTCGTGCAGATCTACGATCCGGA-3′ 3749-3727b
P4 5′-GCGCTGATCGAATTCCTCAT-3′ 2371-2390b
P5 5′-CGACCAGCTGTGGGTCGAAG-3′ 7471-7452b
P6 5′-GTAGTCGGTGTTCATCTGCT-3′ 7236-7217b
P7 5′-AAGCTGAACCTGTACGACAG-3′ 6581-6600b
P8 5′-TTGTAGTTCTCCAGGGTGGG-3′ 6357-6338b
P9 5′-TCAAGAGCTACGACTTCGGC-3′ 5969-5988b
P10 5′-ACGACAGATAGAAGGCCAG-3′ 5350-5332b
P11 5′-GACTTCGCCAAGAAGGATCT-3′ 4784-4803b
P12 5′-GCGAACACCGACTTGTTGTA-3′ 4398-4379b
P13 5′-CTTCATGGTCCGGATCGTATC-3′ 3719-3739b
P14 5′-CCCGGAACATTCGACATCAC-3′ 3782-3801b
P15 5′-GACACCTCGAGCGTCGACCC-3′ 3834-3815b
P16 5′-TCTCGGGCGATCTCGGCAAGTGTGG-3′ 3666-3690b
P17 5′-CGACCCGAATGTATCGCATGTGATG-3′ 3820-3796b
P18 5′-GCGTCGACCCGACTGTTTCGCATGT-3′ 3824-3800b
P19 5′-AGCGTCGACCCGCATGTTTCGCATG-3′ 3825-3801b
P20 5′-CCCGACAGGATGATTGAGACTTG-3′ 1956-1934c
P21 5′-AGGAGACCTTCCCTGTCCTCGTC-3′ 1784-1806c
P22 5′-ACGACGTGAGTGTTGGCCTGAC-3′ 223-202d
P23 5′-CTCTTGAGCTGCGACTAGCCAG-3′ 112-133d
P24 5′-GGCGTCCACGGTACAGACACCC-3′ 1396-1375d
P25 5′-GGCTGAGCGGCGTACGGACAAC-3′ 1263-1284d
P26 5′-CAGCGAGCGGTGGAACGACAAC-3′ 1520-1541c
P27 5′-CGTCACACACCGGCAAACACAA-3′ 1618-1597c
P28 5′-CCTTGTCCTGGACCTTCACC-3′ 4058-4039b
a

The mutagenized restriction sites are underlined.

b

Nucleotide numbering of the genes (accession no. AB110645).

c

Nucleotide numbering of the genes (accession no. AB110643).

d

Nucleotide numbering of the genes (accession no. AB110644).