TABLE 4.
Expression of ParcA subclones
| ParcA promoter construct | Specific β-galactosidase activity (Miller units), at the indicated arginine concna
|
Fold regulation (10/0.1 mM)b | |
|---|---|---|---|
| 0.1 mM | 10 mM | ||
| pILORI4::ParcA-1 | 7.0 (±0.7) | 51.0 (±15.8) | 7.3 |
| pILORI4::ParcA-3 | 32.6 (±5.3) | 97.5 (±1.1) | 3.0 |
| pILORI4::ParcA-4 | 84.1 (±16.6) | 67.0 (±8.0) | 0.8 |
| pILORI4::ParcA-5 | 11.9 (±2.2) | 35.1 (±8.7) | 2.9 |
| pILORI4::ParcA-6 | 16.7 (±1.0) | 37.5 (±8.2) | 2.2 |
| pILORI4::ParcA-8 | 11.8 (±2.3) | 9.6 (±3.1) | 0.8 |
| pILORI4::ParcA-7 | 1.0 (±0.6)c | 0.4 (±0.1)c | 0.4 |
a
Activity was measured in cells from three independent cultures in CDM15 harvested during the transition phase of growth. Standard deviations are shown in parentheses.
b
Specific β-galactosidase activity in CDM15 with 10 mM arginine divided by that in CDM15 with 0.1 mM arginine.
c
Corresponds to the low intrinsic β-galactosidase activity from the empty pILORI4 vector (data not shown).