Figure 2.

mRNA Expression Levels of Genes Regulating Heart Development and Genes within the 22q11 Deletion Interval

(A) Schematic drawing of the genomic context of the Tbx1 gene and genes flanking the locus on chromosome 16 in the mouse, a region syntenic with the 22q11 deletion interval in human.

(B) Schematic representation of major regulators of transcription during heart development.

(C–I) mRNA expression levels as assessed by RT-qPCR and normalized to housekeeping genes in Moz^+/+ and Moz^−/− embryos at E8.5 (C and D), E9.5 (E–H), and E10.5 (I); mRNAs encoded by genes as indicated in the graphs. ^∗p < 0.05 and ^∗∗p < 0.01. Exact p values are given below. Significantly lower in Moz^−/− embryos were mRNAs encoded by Tbx1 at E8.5 (C, p = 0.029), at E9.5 (E, p = 0.001), and E10.5 (I, p = 0.039); Tbx5 at E9.5 (E, p = 0.011) and E10.5 (I, p = 0.006) and Tbx2 at E10.5 (I, p = 0.014). Not significantly changed were the mRNA expression levels of housekeeping genes (D and H), other genes in the 22q11 deletion interval (F), and major regulators of heart development, Gata4 and Nkx2-5 (G). Data are presented and were analyzed as described in the Experimental Procedures. Data were derived from 36 embryos, n = 6 Moz^+/+ and 6 Moz^−/− per gene at each developmental stage.