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IL-1α transcription is up-regulated during hypoxia in A549 epithelial cells.(A) A549 cells were cultured in either normoxic or hypoxic conditions for 12 h. Cells lysates were analyzed by Western blot for IL-1α detection. Bands are marked as precursor or mature. (B) A549 cells were cultured in either normoxic or hypoxic conditions for 6 h, and were analyzed for relative quantification (RQ) of IL-1α transcription. Graph represents mean ± SD of three biological repeats.
