FIGURE 2.

Silencing HIFα proteins down-regulates IL-1α transcription in hypoxic A549 epithelial cells. A549 cells were transfected with HIF-1α siRNA (A) or HIF-2α siRNA (B) 24 h prior to hypoxia (6 h). Each graph represents mean ± SEM of three independent experiments. (C) Cells were transfected with both HIF-1α and HIF-2α mixed siRNA 24 h prior to hypoxia (6 h). Graph represents mean ± SEM of five independent experiments. P values of HIF-2α or HIF-1/2α siRNA-transfected cells vs. control (Ct) siRNA-transfected cells are annotated in the graphs.