FIGURE 3.

Silencing HIFα proteins up-regulates IL-1α transcription in hypoxic WI-38 fibroblasts.(A) WI-38 fibroblasts were cultured in either normoxic or hypoxic conditions for 2, 4, or 6 h, and were analyzed by qRT-PCR for RQ of IL-1α transcription. Graph represents the average ± SEM of two different experiments. RQ of IL-1α transcription in WI-38 cells transfected with HIF-1α (B), HIF-2α (C) siRNA 24 h prior to 6 h culture in hypoxic conditions. Each graph represents mean ± SEM of three independent experiments. (D) Cells were transfected with both HIF-1α and HIF-2α mixed siRNA 24 h prior to hypoxia (6 h). Graph represents mean ± SEM of four independent experiments. P values of HIF-1/2α siRNA transfected cells vs. control (Ct) siRNA-transfected cells are annotated in the graphs.