FIG. 1.

Sarcosine-insoluble fraction of H. pylori 26695 were separated by 2-DE with an IPG strip, followed by SDS-12.5% PAGE. Spots were detected by silver staining. The circled proteins were identified by MALDI-TOF-MS. (A) IPG strip, pH 3.0 to 10.0; (B) IPG strip, pH 6.0 to 11.0. Strain 26695 was grown as described in Materials and Methods, and 300 μg of sarcosine-insoluble fraction was loaded in the first dimension. Identified proteins are indicated by spot numbers in Table 1. Molecular size markers are shown on the left in kilodaltons.