TABLE 1.
List of identified proteins in the sarcosine-insoluble fraction of H. pylori strain 26695
| Spot no. | Annotation | Source or referencea | TIGR locus |
Mr
|
pI
|
No. of matching peptides | Sequence coverage (%) | ||
|---|---|---|---|---|---|---|---|---|---|
| Observed | Theoretical | Observed | Theoretical | ||||||
| 1 | Hypothetical protein | HP0205 | 94,000 | 94,347 | 8.4 | 8.4 | 7 | 11.0 | |
| 2 | OMP (Omp2)b | HP0025 | 77,000 | 77,665 | 9.2 | 9.2 | 17 | 30.0 | |
| 3 | OMP (Omp19) | HP0896 | 75,000 | 77,693 | 8.0 | 8.6 | 5 | 16.0 | |
| 4 | Phosphoglycerate dehydrogenase (SerA) | 10 | HP0397 | 70,000 | 57,906 | 6.5 | 6.7 | 4 | 14.0 |
| 5 | Phosphoglycerate dehydrogenase (SerA) | 10 | HP0397 | 70,000 | 57,906 | 6.6 | 6.7 | 5 | 13.0 |
| 6 | Phosphoglycerate dehydrogenase (SerA) | 10 | HP0397 | 70,000 | 57,906 | 6.7 | 6.7 | 4 | 19.0 |
| 7 | OMP (Omp27) | HP1177 | 69,000 | 69,692 | 9.0 | 9.2 | 11 | 27.0 | |
| 8 | OMP (Omp27) | HP1177 | 69,000 | 69,692 | 9.1 | 9.2 | 4 | 18.0 | |
| 9 | OMP (Omp27) | HP1177 | 69,000 | 69,692 | 9.2 | 9.2 | 7 | 14.0 | |
| 10 | OMP (Omp27) | HP1177 | 69,000 | 69,692 | 9.3 | 9.2 | 5 | 15.0 | |
| 11 | OMP (Omp27) | HP1177 | 69,000 | 69,692 | 9.3 | 9.2 | 6 | 17.0 | |
| 12 | OMP (Omp25) | HP1177 | 66,000 | 76,346 | 9.0 | 9.1 | 5 | 17.0 | |
| 13 | Urease beta subunit (UreB) | 10, 28, 30 | HP0072 | 61,000 | 61,671 | 5.6 | 5.6 | 10 | 23.0 |
| 14 | Catalase | 48 | HP0875 | 59,000 | 58,630 | 8.3 | 8.7 | 14 | 32.0 |
| 15 | Catalase | 48 | HP0875 | 59,000 | 58,630 | 8.4 | 8.7 | 12 | 27.0 |
| 16 | Catalase | 48 | HP0875 | 59,000 | 58,630 | 8.5 | 8.7 | 12 | 30.0 |
| 17 | Catalase | 48 | HP0875 | 59,000 | 58,630 | 8.6 | 8.7 | 14 | 32.0 |
| 18 | Catalase | 48 | HP0875 | 59,000 | 58,630 | 8.7 | 8.7 | 6 | 20.0 |
| 19 | OMP (Omp20) | 29, 35 | HP0912 | 55,000 | 55,932 | 9.0 | 9.1 | 8 | 23.0 |
| 20 | OMP (Omp20) | 29, 35 | HP0912 | 55,000 | 55,932 | 9.1 | 9.1 | 9 | 20.0 |
| 21 | OMP (Omp20) | 29, 35 | HP0912 | 55,000 | 55,932 | 9.2 | 9.1 | 7 | 13.0 |
| 22 | OMP (Omp6) | HP0229 | 55,000 | 55,932 | 9.5 | 9.2 | 4 | 15.0 | |
| 23 | OMP (Omp21) | 29 | HP0913 | 50,000 | 57,063 | 8.0 | 9.2 | 5 | 13.0 |
| 24 | OMP (Omp21) | 29 | HP0913 | 50,000 | 57,063 | 8.4 | 9.2 | 5 | 12.0 |
| 25 | OMP (Omp21) | 29 | HP0913 | 50,000 | 57,063 | 8.5 | 9.2 | 4 | 11.0 |
| 26 | OMP (Omp21) | 29 | HP0913 | 50,000 | 57,063 | 9.0 | 9.2 | 6 | 14.0 |
| 27 | OMP (Omp21) | 29 | HP0913 | 50,000 | 57,063 | 9.2 | 9.2 | 6 | 14.0 |
| 28 | Hypothetical protein | HP1349 | 44,000 | 44,396 | 8.3 | 8.9 | 4 | 14.0 | |
| 29 | Hypothetical protein | HP1349 | 44,000 | 44,396 | 8.3 | 8.9 | 4 | 14.0 | |
| 30 | OMP (Omp32) | HP1501 | 42,000 | 42,976 | 9.5 | 9.5 | 9 | 33.0 | |
| 31 | OMP (Omp32) | HP1501 | 42,000 | 42,976 | 9.5 | 9.5 | 6 | 22.0 | |
| 32 | Hypothetical protein | HP0052 | 39,000 | 39,246 | 9.0 | 8.3 | 6 | 22.0 | |
| 33 | Iron(III) ABC transporter, periplasmic iron-binding protein (CeuE) | 48 | HP1561 | 37,000 | 37,552 | 9.4 | 9.2 | 4 | 19.0 |
| 34 | Iron(III) ABC transporter, periplasmic iron-binding protein (CeuE) | 48 | HP1561 | 37,000 | 37,552 | 9.4 | 9.2 | 4 | 16.0 |
| 35 | Glutamine ABC transporter, periplasmic glutamine-binding protein | HP1172 | 31,000 | 31,222 | 6.2 | 6.9 | 4 | 16.0 | |
| 36 | OMP (Omp24) | HP1113 | 31,000 | 31,896 | 9.2 | 9.2 | 4 | 17.0 | |
| 37 | OMP (Omp4) | HP0127 | 31,000 | 31,890 | 9.5 | 9.6 | 5 | 24.0 | |
| 38 | OMP (Omp4) | HP0127 | 31,000 | 31,890 | 9.6 | 9.6 | 5 | 17.0 | |
| 39 | Amino acid ABC transporter, periplasmic binding protein (Yckk) | HP0940 | 30,000 | 28,737 | 9.3 | 9.4 | 5 | 24.0 | |
| 40 | Amino acid ABC transporter, periplasmic binding protein (Yckk) | HP0940 | 30,000 | 28,737 | 9.4 | 9.4 | 4 | 17.0 | |
| 41 | Putative neuraminyllactose-binding hemagglutinin homolog (HpaA) | 10 | HP0410 | 29,000 | 28,349 | 7.8 | 7.9 | 5 | 23.0 |
| 42 | OMP (Omp10) | HP0324 | 29,000 | 28,767 | 7.8 | 7.8 | 5 | 24.0 | |
| 43 | OMP (Omp15) | HP0706 | 29,000 | 29,929 | 9.1 | 9.0 | 8 | 27.0 | |
| 44 | OMP (Omp15) | HP0706 | 29,000 | 29,929 | 9.2 | 9.0 | 5 | 26.0 | |
| 45 | OMP (Omp15) | HP0706 | 29,000 | 29,929 | 9.3 | 9.0 | 8 | 31.0 | |
| 46 | Urease alpha subunit (UreA) | 29, 48 | HP0073 | 28,500 | 26,540 | 8.3 | 8.5 | 10 | 54.0 |
| 47 | Urease alpha subunit (UreA) | 29, 48 | HP0073 | 28,500 | 26,540 | 8.5 | 8.5 | 6 | 44.0 |
| 48 | OMP (Omp14) | HP0671 | 27,000 | 30,347 | 9.2 | 9.2 | 8 | 36.0 | |
| 49 | OMP (Omp31) | HP1469 | 26,000 | 28,152 | 9.4 | 9.5 | 8 | 37.0 | |
| 50 | OMP (Omp31) | HP1469 | 26,000 | 28,152 | 9.5 | 9.5 | 9 | 44.0 | |
| 51 | Conserved hypothetical secreted protein | HP0139 | 25,500 | 27,492 | 6.6 | 6.9 | 4 | 22.0 | |
| 52 | Flagellar basal-body L-ring protein (FlgH) | HP0325 | 25,000 | 26,469 | 8.5 | 8.7 | 4 | 22.0 | |
| 53 | Alkyl hydroperoxide reductase (TsaA) | 10, 28, 30 | HP1563 | 24,500 | 22,235 | 6.2 | 6.2 | 8 | 50.0 |
| 54 | OMP (Omp23) | HP1107 | 22,000 | 25,698 | 9.1 | 9.1 | 4 | 31.0 | |
| 55 | Membrane fusion protein (MtrC) | HP0606 | 21,000 | 25,957 | 9.2 | 8.8 | 4 | 26.0 | |
| 56 | Glutamine synthetase (GlnA) | 10, 30 | HP0512 | 20,000 | 18,920 | 9.3 | 9.0 | 4 | 30.0 |
| 57 | Hypothetical protein | 9 | HP1173 | 20,000 | 20,586 | 8.5 | 8.9 | 8 | 28.0 |
| 58 | Hypothetical protein | 9 | HP1173 | 20,000 | 20,586 | 9.2 | 8.9 | 4 | 20.0 |
| 59 | Hypothetical protein | 9 | HP1173 | 20,000 | 20,586 | 9.3 | 8.9 | 5 | 42.0 |
| 60 | (3R)-Hydroxymyristoyl-(acyl carrier protein) dehydratase (FabZ) | HP1376 | 18,000 | 18,196 | 6.6 | 6.6 | 4 | 35.0 | |
| 61 | OMP (Omp11) | HP0472 | 17,000 | 20,925 | 9.2 | 9.2 | 6 | 38.0 | |
| 62 | Neutrophil-activating protein (NapA) | 10, 28, 30 | HP0243 | 16,000 | 16,835 | 5.5 | 5.8 | 6 | 46.0 |
a
That is, the reference numbers of previously identified proteins.
b
Refers to the 26695 genome annotation (www.tigr.org).