BMDC were stimulated with plate bound Jagged1-Fc (Jgd1) or IgG1 (Fc control) in the presence or absence of 100 ng/ml LPS. (A) mRNA transcripts were measured, after 4 h, by qRT-PCR. (B) Accumulation of cytokines in the supernatant, after 24 h, was measured by ELISA. Data are mean ± SD of triplicates and are representative of at least 6 independent experiments. Friedman test with Dunn’s post-test was used to statistically compare each stimulus; P<0.05 was considered significant. * = P<0.05, ** = P<0.01, *** = P<0.001, ns = not significant. ND = not detected.