mRNA expression of (A) IL-27 p28, (B) TGFβ1, (C) EBI3, (D) CD3 and (E) Foxp3 were analyzed by quantitative RT-PCR in cardiac untreated rejected allografts (Rejected), treated tolerant allografts (Tolerant) and allografts from RAG −/− recipients harvested at day 2, 5, 7, 8 or 10 after transplantation (n≥2). Results are expressed in mRNA Units/HPRT ratio ± SEM * p<0.05, ** p<0.01.