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Determination of the minimal heme-responsive bhuR promoter region. B. bronchiseptica B013N carrying bhuR-lacZ reporter plasmid pRK41, pRK51, pRK50, or pRK45 was cultured in parallel in iron-depleted SS medium with (hatched bars) or without (open bars) hemin supplementation. Bars represent Miller units of LacZ activity ± 1 standard deviation (n = 3).
