TABLE 1.
Bordetella strains and reporter plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| UT25Sm1 | B. pertussis; spontaneous streptomycin-resistant derivative of wild-type strain UT25 | 19 |
| PM8 | B. pertussis UT25Sm1 ΔhurI | 57 |
| B013N | B. bronchiseptica; spontaneous nalidixic acid-resistant derivative of wild-type B013 | 2 |
| Plasmids | ||
| pRK40 | pRK415 with 3.3-kb EcoRI-HindIII trp′-′lacZ insert fragment; Tetr | 57 |
| pRK41 | pRK40 with 0.5-kb B. pertussis UT25 ′hurR-bhuR′ insert fragment; bhuR-lacZ transcriptional fusion; Tetr | 57 |
| pRK42 | pRK40 with 2.1-kb B. pertussis UT25 hurIR-bhuR′ insert fragment; hurIR bhuR-lacZ transcriptional fusion; Tetr | 57 |
| pRK45 | pRK40 with 0.3-kb B. pertussis UT25 bhuR′ insert fragment; bhuR-lacZ transcriptional fusion; Tetr | This study |
| pRK47 | pRK42 with 12-nt block substitution in bhuR promoter region; hurIR bhuR-lacZ transcriptional fusion; Tetr | This study |
| pRK48 | pRK42 with unique BglII site in hurR-bhuR intergenic region; hurIR bhuR-lacZ transcriptional fusion; Tetr | This study |
| pRK49 | pRK48 with Ω Cm cassette inserted into BglII site, hurIR Ω Cm bhuR-lacZ transcriptional fusion; Tetr | This study |
| pRK50 | pRK40 with B. pertussis UT25 0.38-kb bhuR′ insert fragment; bhuR-lacZ transcriptional fusion; Tetr | This study |
| pRK51 | pRK40 with B. pertussis UT25 0.44-kb ′hurR-bhuR′ insert fragment; bhuR-lacZ transcriptional fusion; Tetr | This study |