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. Author manuscript; available in PMC: 2013 Mar 6.
Published in final edited form as: Nature. 2012 Sep 6;489(7414):115–120. doi: 10.1038/nature11376

Figure 4. The same interfaces in E2 and ubiquitin are important for CHIP and RNF4 activity.

Figure 4

a, Autoubiquitylation activity of CHIP (upper panel) and RNF4 (lower panel) with ubiquitin mutants. Western blots probed with anti-ubiquitin antibody are shown. Longer exposure is shown for I36A and L71A ubiquitin, as binding of the antibody is affected by these mutations. b, Autoubiquitylation activity as in a, but with UbcH5a mutants.