Figure 6. Sox9 is dispensable for adult prostate regeneration.

A. Timeline for serial prostate regression-regeneration assay in castrated mice. Castration regeneration experiments were carried out with seven sets of UGS grafts. Both ER-Sox9^+/+ and ER-Sox9^flox/+ tissue were used as controls with no difference seen. Castration regeneration was also carried out for two rounds of hormone replacement with subcutaneous (vs oral) DHT (50mg/kg) with yielded similar results (Data not shown). B. Gross images of grafts after 10 months. C. Cycled grafts were examined for Sox9, Nkx3.1, p63, CK18, AR, and CK5 expression by IHC. Scale bars = 100 μM