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Triple fluorescence micrographs for c-Fos (blue; a, e, i, and m), PAG (magenta: c and g), TH (magenta; k and o), and fluorogold (green; b, f, j, and n) for WT and DAT KO mice treated with nisoxetine (10 mg/kg, i.p.) after fluorogold injection into the NAc. PAG (phosphate-activated glutaminase): glutamatergic neuronal marker and TH (tyrosine hydroxylase): dopaminergic marker. Scale bars, 100 μm.
