Table 1. Primer Sequences used for Semi-Quantitative Real-Time PCR.

Gene	Gene bank	Primer sequences (5′–3′)	Amplicon size
HPRT	NM_012583.2	Fwd GACCGGTTCTGTCATGTCG	61
		Rev ACCTGGTTCATCATCACTAATCAC
GAPDH	XR_009170.1	Fwd AGGTCAGTGTGAACTGATTTGGT	61
		Rev GAGTAGAAGGCAGCCCTGGT
D2R	NM_012547	Fwd AAGCGCCGAGTTACTGTCAT	111
		Rev GGCAATGATACACTCATTCTGGT
D3R	NM_007877	Fwd CAGACCTGGCTTCCCTCAG	71
		Rev TGGCCCTTATTGAAAACTGC
CB1R	NM_012784	Fwd GGACATGGAGTGCTTTATGATTC	64
		Rev GAGGGACAGTACAGCGATGG
TH	NM_012740.3	Fwd TCTCCCTGAGGGGTACAAAA	75
		Rev GAATTTTGGCTTCAAATGTCTCA

The table lists 19–24 bp forward (Fwd) and reverse (Rev) primer sequences for the appropriate gene, along with the expected PCR amplicon size for each primer sequence.