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. 2012 Aug 14;1(8):e37. doi: 10.1038/mtna.2012.28

Figure 2.

Figure 2

Higher total flow rates through the staggered herringbone micromixer (SHM) reduce lipid nanoparticle (LNP) small interfering RNA (siRNA) polydispersity. Dependence of the polydispersity index (PDI) on the total flow rate and the concentration of lipid and siRNA in the ethanol and aqueous phases, respectively. The PDI was determined from the second order coefficient in the cumulants analysis provided by dynamic light scattering (DLS) (PDI = (σ/µ)2). The total flow rate was varied from 0.02 to 4 ml/min keeping the aqueous buffer to ethanol volumetric flow rate ratio constant at 3:1. The aqueous siRNA concentration was varied from 0.25 to 0.59 mg/ml while the lipid concentration was varied from ~4 to 10 mg/ml to keep the siRNA/total lipid ratio constant at 0.06 wt/wt. PDI values represent averages from 4 measurements. The lipid composition employed was DLinKC2-DMA/DSPC/cholesterol/PEG-c-DMA at mol ratios of 40:11.5:47.5:1. The aqueous buffer was 25 mmol/l acetate, pH 4. DSPC, 1,2-distearoyl-sn-glycero-3-phosphocholine.