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. 2012 Aug 14;1(8):e37. doi: 10.1038/mtna.2012.28

Figure 5.

Figure 5

Cryo-transmission electron microscopy (cryo-TEM) micrographs of lipid nanoparticle (LNP) small interfering RNA (siRNA) systems containing 1 and 5 mol% PEG-c-DMA. (a) Cryo-TEM micrograph of LNP siRNA composed of DLinKC2-DMA/DSPC/cholesterol/PEG-c-DMA (mol ratios 40:11.5:47.5:1) and siRNA at an siRNA to total lipid ratio 0.06 (wt/wt). (b) Cryo-TEM micrograph of LNP siRNA composed of DLinKC2-DMA/DSPC/cholesterol/PEG-c-DMA (mol ratios 40:11.5:43.5:5) and siRNA at an siRNA-to-total lipid ratio 0.06 (wt/wt). LNP were imaged at 50K magnification. LNP formulation was performed under rapid mixing conditions (4 ml/min total flow rate with an siRNA-buffer:lipid–ethanol volumetric flow rate ratio of 3:1) with the staggered herringbone micromixer (SHM), with an ethanol phase containing 30 mmol/l lipid. The LNP siRNA dispersion was concentrated before imaging. The scale bar represents 100 nm. DSPC, 1,2-distearoyl-sn-glycero-3-phosphocholine.