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. Author manuscript; available in PMC: 2012 Sep 14.
Published in final edited form as: Neuron. 2009 Feb 26;61(4):527–540. doi: 10.1016/j.neuron.2009.01.010

Table 5.

List of Stealth® RNAi sequences

Stealth RNAi (sense strand) Control RNAi (sense strand)
1. 994 (start site of the RNAi)
GAC UAC AUC UGU ACU GGC GUC AAU A		 1. Control 994
GAC CUA CAU GUG GUC CUG CAA UAU A
2. 2737 (start site of this RNAi)
GGU AAC GGA GUA CUG GUC AAC UAC U		 2. Control 2737
GGU AGG CCA UGU GGU CAA CUA AAC U
3. 5072 (start site of this RNAi)
CGU CCU UGA UGA ACG ACC UUG ACA A		 3. Control 5072
CGU AGU UAA GUC AGC GUU CAC CCA A

Note: Stealth RNAi sequences were designed based on the algorithm provided by Invitrogen, CA. This table shows only the sense strand of the RNAi duplex. To determine the effects of these RNAi during de novo formation of SN-MN synapses, a mixture of these RNAi (6.7 μM) was microinjected into Aplysia neurons at 6 h after plating the cells in vitro and thereafter every 48 h. To determine the effect of these RNAi on the expression of LTF, a mixture of these RNAi was microinjected into mature Aplysia neuronal culture on day 4. Cells were then stimulated with 5-HT to induce facilitation.