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. Author manuscript; available in PMC: 2012 Sep 14.
Published in final edited form as: Cell. 2011 Dec 9;147(6):1369–1383. doi: 10.1016/j.cell.2011.09.056

Figure 1. Neurl1 is expressed in the adult mouse forebrain and is localized in dendrites and at post-synaptic sites in hippocampal neurons.

Figure 1

(A) In situ hybridization analysis of Neurl1 mRNA expression on coronal (1&2) and sagittal (3) brain slices from adult (3 ½ months) wild type mice. cx: cerebral cortex, hp: hippocampus, st: striatum, am: amygdala.

(B & C) Confocal sections of adult CA1 pyramidal neurons. Coimmunostaining for Neurl1 and the dendritic marker MAP2 or the presynaptic marker Synaptophysin (Syn). Lower panels: high magnification of apical dendrites.

(D) Immunoblots of hippocampal homogenates (H) and PSD extracts. Neurl1 and PSD95 are enriched in the PSD fraction. Synaptophysin, the glial protein GFAP and histone 3 are controls.

(E) Confocal sections of cultured hippocampal neurons (16 DIV) showing colocalization of endogenous Neurl1 and PSD95. (ii &iii) high magnification of dendrites.

(F) Neurl1 protein level in the CA1 area is increased 30min after LTP induction at the Schaffer collateral pathway using 4 TBS. ctrl: unstimulated controls. Top: examples of western blots from 3 independent experiments. Bottom: Mean + SEM from 5 independent experiments (4 mice; *p<0.01). See also Figure S1.