FIGURE 1.

Syx contributes to protein stability of Rnd3. A, schematic represents Syx construct used in 293T cell expression studies. B, 293T cells were transfected with Rnd plasmids in the absence or presence of Syx(1–800), lysed, and immunoblottted (WB). C, 293T cells were co-transfected with 0.5 μg of GFP-FLAG and 0.5 μg of the indicated plasmids. After 24 h, cells were incubated with cycloheximide (20 μg/ml) and then harvested at the indicated times with equal amounts of total protein loaded (40 μg/lane). Expression of GFP is marked by an asterisk (*). D, 293T cells were co-transfected with 0.5 μg of Rnd3 and 0.5 μg of Syx(1–800) plasmids and treated for the indicated times with cycloheximide, lysed, and immunoblottted. E, in the graphical representation, the y-axis corresponds to the ratio of Rnd3 relative to time 0. The average of three independent experiments is shown. Error bars, S.D.