FIGURE 10:

Translation persists in PKR-knockout MEFs despite the presence of G3BP-induced stress granules. (A) Cells were transfected with G3BP-GFP-λN and either left untreated (no Ars) or treated (+Ars) with arsenite as described in Materials and Methods. RPA was then performed to detect translating ribosomes (α-puromycin; red). (B) Schematic illustration of G3BP-induced granule formation and subsequent eIF2α phosphorylation by PKR. G3BP induction of both small and large SGs is illustrated with arrows toward both sizes of granules. Points of the pathway that have not been further elucidated are depicted with question marks.