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eIF2α mutant S51A MEFs are capable of forming G3BP-induced stress granules. Both S51 and S51A eIF2α mutant MEFs were transfected with G3BP-GFP-λN. Transfected S51 and S51A MEFs were either left untreated (−) or treated for 30 min with 200 μM arsenite (+) followed by fixation and staining for Tia1 before deconvolution imaging.
