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. 2012 Sep 15;23(18):3532–3541. doi: 10.1091/mbc.E12-06-0429

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© 2012 Yao et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Confocal time-lapse analysis of Chromator-GFP during mitosis in syncytial Drosophila embryos. (A) Relative dynamics of Chromator-GFP (green) and tubulin-mCherry (red) during a complete mitotic cycle. Scale bar, 10 μm. (B) Chromator-GFP at metaphase. Arrowheads indicate the gap between Chromator-GFP's spindle matrix and centrosomal localization. Scale bar, 10 μm. (C) Relative localization of Jupiter-GFP (green) and tubulin-mCherry (red) at metaphase. Scale bar, 5 μm. (D) Relative localization of Chromator-GFP (green) and tubulin-mCherry (red) at metaphase. Scale bar, 5 μm. (E, G) Line-scan plots of pixel intensity across the spindle along the white lines in C and D for Jupiter-GFP/tubulin-mCherry and Chromator-GFP/tubulin-mCherry, respectively. The images in C and D are both from a single confocal optical plane. The asterisks indicate the likely position of microtubule K-fibers. (F, H) Plots of the correlation between pixel intensity between Jupiter-GFP/tubulin-mCherry and Chromator-GFP/tubulin-mCherry across the spindle along the white lines in C and D, respectively. The regression line and the value of Pearson's coefficient are indicated for each plot.