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. 2012 Sep 15;23(18):3532–3541. doi: 10.1091/mbc.E12-06-0429

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© 2012 Yao et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — Spindle matrix dynamics after colchicine injection before nuclear envelope breakdown. (A) Two image panels from the beginning and end of a time-lapse sequence of Chromator-GFP (green) and tubulin-mCherry (red) after colchicine injection. (B) Two image panels from the beginning and end of a time-lapse sequence of Chromator-GFP (green) and histone H2Av-RFP (red). (C) Plot of the average pixel intensity in regions of interest (ROIs) outside the nucleus (red) and inside the nucleus (blue) as a function of time in a colchicine-injected embryo. The two image inserts correspond to the area outlined by a white boxes in A before and after NE breakdown, respectively. The ROIs are indicated by white squares. The difference in expression levels of Chromator-GFP in A and B is due to use of high- and low-expression driver lines, respectively.