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. 2012 Sep 15;23(18):3532–3541. doi: 10.1091/mbc.E12-06-0429

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© 2012 Yao et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Time-lapse analysis of the spindle matrix protein Megator in syncytial embryos. (A) Relative dynamics of full-length Megator-YFP (Mtor-FL) and histone H2Av-RFP (H2Av) during a complete mitotic cycle. The images show their distribution at interphase 1, metaphase, and interphase 2, respectively. The diagram beneath the images shows the domain structure of Megator with the coiled-coil region in black, the CTD in white, and the endogenous NLS in red. Scale bar, 20 μm. (B) Relative dynamics of a truncated, GFP-tagged, carboxy-terminal construct of Megator (Mtor-CTD) and histone H2Av-RFP (H2Av) during a complete mitotic cycle. The images show their distribution at interphase 1, metaphase, and interphase 2, respectively. Mtor-CTD is diagrammed below the images. Scale bar, 20 μm. (C) Relative dynamics of a truncated, GFP-tagged, amino-terminal construct of Megator (Mtor-NTD) and histone H2Av-RFP (H2Av) during interphase and metaphase. Mtor-NTD is diagrammed below the images. Scale bar, 10 μm. (D) The localization patterns of Mtor-FL, Mtor-NTD, and Mtor-CTD at interphase. Mtor-FL localizes to the nuclear interior, as well as to the nuclear rim, Mtor-NTD is present at the nuclear rim with no or very little interior nuclear localization, and Mtor-CTD is diffusively present in the nucleoplasm without detectable nuclear rim localization. (E) Top, three images from a time-lapse sequence of Mtor-FL-YFP (green) and histone H2Av-RFP (red) after colchicine injection at interphase. Middle, three images from a time-lapse sequence of Mtor-CTD-GFP (green) and histone H2Av-RFP (red) after colchicine injection at interphase. Bottom, three images from a time-lapse sequence of Mtor-NTD-GFP (green) and histone H2Av-RFP (red) after colchicine injection at interphase. Time is in minutes and seconds. Scale bars, 10 μm.