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. 2012 Sep 15;23(18):3647–3662. doi: 10.1091/mbc.E12-04-0334

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© 2012 Monteiro et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — The kinesin KLP-4 functions in the VNC to regulate the abundance of GLR-1 and GLR-1–dependent behavior. (A) Protein domain organization of C. elegans KLP-4, Drosophila Khc-73, and mouse KIF13A and KIF13B. The N-terminal motor domain (red box) containing the signature ATP-binding motif (thin black bar) and microtubule-binding regions (thick black bar), the cargo-binding domain (gray box), the Forkhead-associated domain (FHA; purple box), and the microtubule plus end–binding domain (CAP-Gly; orange box) are shown. The percentage identity of the motor domain of C. elegans KLP-4 (percentages in white) or full-length KLP-4 (percentages in black) vs. Drosophila Khc73 and mouse KIF13A and KIF13B are indicated. (B–F) Representative images of the anterior VNC of larval stage 4 (L4) wild-type (B), klp-4(pz19) (C), rescued klp-4(pz19) (D), klp-4(tm2114) (E), and rescued klp-4(tm2114) (F) animals expressing an integrated GLR-1::GFP transgene (nuIs25). In these and all subsequent images anterior is to the left and ventral is up. (G, H) Quantification of GLR-1::GFP puncta intensities (normalized; G) and densities (H) for the strains pictured in B–F. Means and SEM are shown for n = 38 wild-type, n = 23 klp-4(pz19), n = 21 rescued klp-4(pz19), n = 20 klp-4(tm2114), and n = 21 rescued klp-4(tm2114) animals. (I) Quantification of number of spontaneous reversals per minute for n = 20 wild-type, n = 20 klp-4(tm2114), and n = 23 animals expressing an integrated klp-4 transgene under the control of the glr-1 promoter (klp-4(xs)) (pzIs20), n = 14 glr-1(n2461), and n = 13 glr-1(n2461) animals overexpressing klp-4 (klp-4(xs)). Values that differ significantly from wild type are indicated by asterisks above each bar, whereas other comparisons are marked by brackets (**p ≤ 0.001, *p ≤ 0.01, ^#p ≤ 0.05, Tukey–Kramer test).