FIGURE 3:

Enzymes that produce phosphatidic acid promote Bazooka activity but not localization in vivo. (A) Categories of cuticle phenotypes scored in experiments. (B–D) Quantification of phenotypic ranges from specific crosses shown. Each data set is an average of two separate experiments (N = 98–406 embryonic cuticles per experiment). (B) Overexpression of DGK partially rescues the baz mutant cuticle phenotype, but expression of PLD has minimal effect. baz^Xi106, gal4 is short form for baz^Xi106, maternal-α4-tubulin-GAL4-VP16. (C) Reducing the levels of DGK (rdgA encodes DGK) or PLD enhances the baz^Xi106 mutant cuticle phenotype. rdgA^KS60 and Pld^Null alleles were used. (D) Combined reduction of DGK and PLD enhances the baz^Xi106 mutant cuticle phenotype further. Combined reduction of DGK and PLD also enhances the baz^GD21 mutant cuticle phenotype vs. an outcrossed control. rdgA^KS60 and Pld^Null alleles were used in each case. (E) Staining for Baz in baz zygotic mutants vs. their heterozygous siblings with or without DGK overexpression shows that the partial rescue of the baz mutant cuticle phenotype with DGK overexpression is not due to a detectable stabilization of the maternal supply of Baz protein around the apical cortex. Each image was collected and adjusted with the same settings. Histograms below show the numbers of pixels for each grayscale value for images collected with the same settings. The pixel numbers are averages for the sample sizes indicated for each genotype. Note the higher-intensity values forming shoulders in the heterozygous sibling distributions and the similar shapes of the hemizygous mutant distributions with or without DGK overexpression.