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. 2012 Jul 26;287(38):31973–31982. doi: 10.1074/jbc.M112.348565

FIGURE 3.

FIGURE 3.

Functional consequence on Gs signaling in the B2AR of Ala substitutions of the ArgIII:26/3.50 micro-switch and its potential interaction partners. A–D, basal and agonist (pindolol)-induced cAMP production in COS-7 cells transiently transfected with either WT β2-AR (dotted line) or mutant forms of the following: AspIII:25Ala (A), GluVI:-06Ala (B), TyrV:24Ala (C), and ArgIII:26Ala (D). % of max., percentage of maximum. E and F, isoproterenol-induced cAMP production in CHO-K1 cells transiently transfected with either WT (dotted line) or mutant forms of the β2-AR: TyrV:24Ala (E) and ArgIII:26Ala (F). Cell surface receptor expression, measured by enzyme-linked immunosorbent assay, is shown in the inserted column diagrams in each panel. Error bars indicate S.E.