FIGURE 1.

CD151 facilitates molecular association of CD151-β₁ integrin complexes with Ras, Rac1, and Cdc42. A and B, stable MelJuSo mock and CD151 transfectant cells (A) and parental C8161 cells (B) were lysed with Brij 97, and immunoprecipitation (IP) was performed with mouse normal IgG, anti-CD151, anti-β₁ integrin, anti-EWI-2, anti-caveolin-1, or anti-MSH-R Abs. The immunoprecipitates were analyzed by immunoblotting (IB) using anti-pan-Ras, anti-RhoA/B/C, anti-Rac1, anti-Cdc42, anti-EWI-2, or anti-Gα_i-2 Abs. C, C8161 cells were transiently transfected with control or CD151-targeting siRNAs, and the CD151 and β₁ integrin immunoprecipitates were examined for the presence of Ras, Rho, Rac1, and Cdc42 by immunoblotting analysis.