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. 2012 Jul 25;287(38):32027–32039. doi: 10.1074/jbc.M111.314443

FIGURE 6.

FIGURE 6.

CD151-mediated activation of Ras and Rac1 is dependent on FAK and Src activities involved in integrin signaling. A, empty vector- and CD151-transfected 293 cells were cultured onto plates precoated with laminin (LN) or poly-l-lysine (pL) for 24 h. After treating cells with membrane fragments of MelJuSo mock (M) or CD151 (CD) transfectant cells for 60 min, phosphorylation levels of FAK and Src in cell lysates were assessed by immunoblotting analysis using Abs recognizing phospho-FAK(Tyr-925) and phospho-Src(Tyr-416). Numbers below the immunoblot indicate relative ratio of phospho-FAK/total FAK or phospho-Src/total Src. B and C, C8161 cells cultured on laminin (LN) or poly-l-lysine (pL) were transfected with control or FAK-targeting siRNA (B) and the expression vectors encoding the constitutively active (CA) or dominant negative (DN) mutant of Src (C). Following treatment of cells with membrane fragments from mock (M) or CD151 (CD) transfectant MelJuSo cells, GTP-loaded Ras and Rac1 proteins were examined in the same manner as in Fig. 5.