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. 2012 Jul 26;287(38):32136–32146. doi: 10.1074/jbc.M112.385096

TABLE 1.

Summary of results for disulfide cross-linked cysteine pairs R334C/T1122C, R334C/G1127C, and T338C/S1118C

Cysteine pairs were studied with either the native Glu present at position 1371 (Glu-1371) or with Gln substituted at this position (Gln-1371). As described under “Discussion,” Glu-1371 is expected to reflect both open and closed channels, and Gln-1371 reflects almost exclusively open channels. In parentheses are indicated the figures in which the quantitative evidence justifying each nonquantitative statement in the table is presented.

R334C/T1122C
R334C/G1127C
T338C/S1118C
Glu-1371 Gln-1371 Glu-1371 Gln-1371 Glu-1371 Gln-1371
Spontaneous cross-linking No (Figs. 1 and 3) Yes (Fig. 6) Yes (Figs. 1 and 3) No (Fig. 6) No (Figs. 1 and 3) No (Fig. 6)
CuPhe-induced cross-linking Yes (Fig. 1) Yes (Fig. 4) Yes (Fig. 1) No (Fig. 4) Yes (Fig. 1) Enhanced (Fig. 4)
Cd2+ inhibition Strong (Fig. 3) Strong (Fig. 5) Moderate (Fig. 3) Abolished (Fig. 5) Weak (Fig. 3) Strengthened (Fig. 5)