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. 2012 Jul 12;287(38):32172–32179. doi: 10.1074/jbc.M112.393462

FIGURE 1.

FIGURE 1.

Generation and characterization of PRL2 knock-out mice. A, structure of the mouse PRL2 locus and the mutant allele containing the pGT01 gene trap cassette. The exons and the initiation codon (ATG) of PRL2 are indicated. Lac Z and neo: expression units of the β-galactosidase and neomycin resistance genes. pA, polyadenylation signal. SA, splice acceptor. Solid arrows indicate primer sites for PCR genotyping. B, representative PCR amplification fragments using primers indicated in A for genotyping. C, quantitative RT-PCR analysis of PRL2 mRNA expression in tissues from PRL2+/+, PRL2+/−, and PRL2−/− mice. n = 3 per group. D, Western blot analyses of PRL2 protein levels in skeletal muscle from PRL2+/+, PRL2+/−, and PRL2−/− mice. E, quantitative RT-PCR analysis of PRL1, PRL2, and PRL3 mRNA expression in E16.5 embryos with PRL2+/+, PRL2+/−, or PRL2−/− genotypes (n = 3 per genotype). The results were presented as the percentage of the wild type ± S.E.