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. 2012 Jul 12;287(38):32172–32179. doi: 10.1074/jbc.M112.393462

FIGURE 6.

FIGURE 6.

PRL2 overexpression enhanced EGF- and IGF2-induced Akt activation in HEK293 cells. A, proliferation rate of HEK293-PRL2 stable cell lines (PRL #1 and #2) and vector control cells. Cell numbers were determined after a 72-h culture by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay measured as optical density at 540 nm (OD 540). Data represent mean of three experiments ± S.E. *, p < 0.01. B, migration rate of HEK293-PRL2 and vector control cells determined by the Transwell migration assay. Cells that migrated to the lower chamber were collected and counted with a hemocytometer. Data represent mean of three experiments ± S.E. *, p < 0.001. C, Western blot showing FLAG-tagged PRL2 expression and Akt phosphorylation in HEK293-PRL2 cells. pAkt473, phospho-Akt473. D, responsiveness of HEK293-PRL2 and vector control cells to EGF and IGF2. Cells were serum-starved overnight, stimulated with 2 ng/ml EGF or 5 ng/ml IGF2 for the indicated times, lysed, and subjected to Western blot for phosphorylated Akt and total Akt signals. pAkt473, phospho-Akt473.